1 AIT Asian Institute of Technology

Low calorie beer making using pullulanase extracted from polished rice in the mashing process and yeast with high fermentation ability

AuthorWang, Xiaohong
Call NumberAIT Thesis no.AE-91-21
Subject(s)Brewing industry

NoteA thesis submitted in partial fulfillment of the requirements for the Degree of Master of Engineering
PublisherAsian Institute of Technology
AbstractThe selected of the suitable yeast strain Saccbaromyces cereyisiae from different sources were investigated in terms of the growth rate and the fermentation ability. The pullulanase enzyme that extracted from rice was added to wort for improve the hydrolyses of the dextrin to fermentable sugar. Two type of enzyme glucoamylase and a-amylase were a l so used during mashing process. Decoction mashing process was used. Mashing condition was tested by using Orthogonal method to find the best combination of different factors for the most fermentable sugar during mashing process. Four f actors each with three l eve l s were tested such as the percentage of rice at 20%, 25% and 30 %, the percent age of sugar at 0%, 5% and 8%, the usage of a - amylase a t 2, 4 and 8 u/g, the usage of glucoamylase at 100, 150 and 200 u/g. Dry beer was made by using laboratory scale fermenter, and the quality was tested. The yeast that uses 2 hours in the l ag phase time and the generation time at 7.6 hours a t 10 °c and gave the maximum fermentation degree of 84.9% was selected for dry beer making. It was found that the wort ma d e by using rice at 25%, pullulanase at 8 u/g, sugar at 8%, a-amylase at 4 u/g, glucoamylase at 150 u/g contains high fermentable sugar. The dry beer from this process was contained the l ow calorie (290-330 Cal) when compared which the regular beer (about 440 Cal).
Year1991
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSAgricultural and Food Engineering (AE)
Chairperson(s)Athapol Noomhorm;
Examination Committee(s)Ilangantileke, S.G.;Jindal, Vinod Kumar;
Scholarship Donor(s)Federal Republic of Germany (DAAD);
DegreeThesis (M.Eng.) - Asian Institute of Technology, 1991


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