Study of Fas pre-association and screening of chemical compounds that can affect fas pre-association | |
| Author | Nguyen Thi Minh Huyen |
| Call Number | AIT Thesis no.FB-05-23 |
| Subject(s) | Apoptosis Cell death |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-05-23 |
| Abstract | Fas (CD95; APO-1) is a member of the TNF receptor family which mediates apoptosis through the binding of Fas Ligand to Fas. The Fas protein is a 317-amino acid type I transmembrane glycoprotein with three extracellular cysteine-rich domains (CRDs) that are characteristic of the tumor necrosis factor receptor (TNFR) superfamily. The Fas cytoplasmic portion contains a death domain that rapidly recruits the adaptor molecule FADD by homophilic interaction and lead to caspase activation and apoptosis. FasL or anti-Fas monoclonal antibody associates with Fas and induces the Fas trimerization causing signal transduction via this trimerization. Fas was previously thought to be oligomerized after binding of its ligand but recently it was found to be pre-associated in living cells. This pre-association is contributed by the cysteine-rich domain I (CRDI) also called Pre-Ligand Assembly Domain (PLAD), required for efficient signaling and dominant interference in human disease. The binding pocket for FasL is major in CRD2 and CRD3 of Fas. The purpose of this study is to analyse the effect of chemical in the interaction of preassociation of Fas extracellular domain. The chemicals that can inhibit or promote the preassociation of' Fas can be used like a drug for preventing or inducing apoptosis in some diseases. This study will help in the process of drug discovery and in which in vitro experiments will be done to screen chemical library in order to find out chemicals, which have profound effects. The results can be further used for in vivo studies |
| Year | 2005 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Kim, Key-Sun; |
| Examination Committee(s) | Rakshit, Sudip Kumar ;Sook Yoo, Young; |
| Scholarship Donor(s) | Korea Institute of Science and Technology ;Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2005 |