Binding affinity and catalytic activity of anti-DNA autoantibody and its cytotoxicity against tumor cell lines | |
| Author | Subedi, Suresh |
| Call Number | AIT Thesis no.FB-04-11 |
| Subject(s) | DNA antibodies Catalysts |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-04-11 |
| Abstract | Anti-DNA autoantibody, that hydrolyzes the DNA of the individual in which it is
produced, ath·act the attention of researches since their discovery. In this study, a monoclonal
anti-DNA autoantibody (G-1-2) was produced and purified from the hybridoma cell line G-1-2.
It's binding affinity and catalytic activity to different forms of DNA was observed. Kinetic
screening of anti-DNA autoantibody (G-1-2) using Surface plasmon resonance (SPR)
technique showed its equilibrium dissociation constant (.KD) with oligonucleotides in
nanomolar (nM) range. The equilibrium dissociation constant (KD) for |
| Year | 2004 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-04-11 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Other Field of Studies (No Department) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip K.; Yu, Jaehoon |
| Examination Committee(s) | Athapol Noomhorm; Jindal, Vinod K. |
| Scholarship Donor(s) | Government of Japan |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2004 |