Fractionation of n-3 PUFA from Tuna fish oil using enzymatic and physical separation methods | |
| Author | Prak Krisna |
| Call Number | AIT Thesis no. BP-00-11 |
| Subject(s) | Fish oils in human nutrition Unsaturated fatty acids in human nutrition |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. BP-00-11 |
| Abstract | Preparation of n-3 polyunsaturated fatty acid (PUFA) concentrates from semi-refined tuna fish oil in the form of acylglycerols was carried out by hydrolysis with a number of commercial microbial lipases. The lipases tested were F-APl 5 from Rhizopus oryzae, Novozyme SP-435 form Candida antarctica and papain from C. papaya. After hydrolysis of oil, free fatty acids were removed, and glyceride fraction containing mono-, di-, and triacylglycerols was determined. Novozyme SP-435 and papain show no specificity on hydrolysis to concentrate n-3 PUF A. F-APl 5 lipase was effective in increasing the amount of n-3 PUF A in the remaining acylglycerols of tuna fish oil. After using different enzyme loads (0.5, 1.0, 3.0, 5.0 and 10.0%) at 39°C, 160rpm and oil:water (1:1 wt./vol) it was found that 0.5% enzyme and 2 hours of hydrolysis is the optimum condition for concentration of DHA in acylglycerols form from 25.2% to 35.3%. However, these enzymes cannot concentrate EPA. Several other methods such as urea complexation, low temperature crystallization and chitosan absorption were attempted to concentrate PUF A from different lipid types obtained from tuna like FFA, GLF and semi-refined oil. FFA with urea (20%, wt./vol.) in 150 ml 90% aqueous ethanol showed the best result for concentration of PUFA. 60.8% DHA and 13.3% EPA were obtained in the noncomplexed fraction of the oil. Though methanol was seen to be slightly better, as a solvent, ethanol is preferred. This is because methanol is toxic for food and medical applications. F-AP15 showed better of reesterification than Novozyme to incorporate PUFA (60.8% DHA and 13.3% EPA) into GLF (34.7%DHA and 6.3%EPA). About 45 .5%DHA and 8.2%EPA (using GLF) fraction of partially hydrolyzed oil, and 34.9%DHA and 8.2%EPA using semi-refined oil in GLF fraction was obtained after reesterification using F-APl 5 enzyme. |
| Year | 2000 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BP-00-11 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Rakshit, Sudip K.;Pioch, Daniel; |
| Examination Committee(s) | Stevens, Willem F.;Pakom Nuchnoi;Suwalee Chandrkrachang; |
| Scholarship Donor(s) | Asian Development Bank Japan Scholarship Program; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2000 |